DNA

Part:BBa_K3878017:Design

Designed by: Louise Merle   Group: iGEM21_UParis_BME   (2021-09-27)


Toehold_miR21-5p_6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 149
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 276


Design Notes

This part can be used with golden gate assembly into a plasmid coding for a repressor gene followed by a T7 terminator (BBa_K3453101).


Source

ordered from IDT

References